A complement-dependent enzyme immunoassay (C-EIA) with increased sensitivity for IgM-rich rabbit sera.

H M Kuhn; L Brade; H Brade

doi:10.1016/0022-1759(92)90286-3

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A complement-dependent enzyme immunoassay (C-EIA) with increased sensitivity for IgM-rich rabbit sera.

H M Kuhn·L Brade·H Brade

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Abstract

An enzyme immunoassay involving activation of complement (C-EIA) was developed for rabbit polyclonal IgM antibodies against lipid A and lipopolysaccharide antigens. C-EIA was significantly higher in sensitivity for IgM-rich rabbit sera compared to EIA using anti-immunoglobulin secondary antibodies. Hence, C-EIA should be useful for the detection of weak IgM reactivities in rabbit sera, especially after short-time immunizations. Selective inhibition of both complement pathways indicated that C-EIA measures activation of the classical pathway.

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Animals Antibodies, Monoclonal Complement Activation Immunoenzyme Techniques Immunoglobulin M Lipopolysaccharides Rabbits Journal Article Research Support, Non-U.S. Gov't

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H M Kuhn, L Brade, H Brade. 1992-11-05. A complement-dependent enzyme immunoassay (C-EIA) with increased sensitivity for IgM-rich rabbit sera.. https://doi.org/10.1016/0022-1759(92)90286-3

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