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PubMed · 1433269

Simian immunodeficiency virus vectors: replication and pseudotyping.

T A Rizvi·A T Panganiban
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Abstract

We studied a single round of replication of Simian immunodeficiency virus (SIV) through the use of a replication defective vector that expresses the hygromycin resistance gene. It was possible to pseudotype SIV particles by complementation with the env gene from a murine amphotropic retrovirus. Moreover, SIV RNA was packaged and propagated by core particles of the heterologous lentivirus, HIV-1. These results indicate that coinfection of cells with SIV and other retroviruses could lead to infection of new cell types in nature.

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AnimalsBlotting, SouthernCell LineCinnamatesDNA, ViralDrug Resistance, MicrobialGene Expression Regulation, ViralGenes, envGenetic VectorsHeLa CellsHumansHygromycin BNucleic Acid HybridizationProvirusesSimian Immunodeficiency VirusTransfectionVirus ReplicationJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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T A Rizvi, A T Panganiban. Simian immunodeficiency virus vectors: replication and pseudotyping.. https://pubmed.ncbi.nlm.nih.gov/1433269/

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