PubMed · 14404178
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H KALOUD, W MARESCH. 1959-09-05. [ABO incompatibility].. https://pubmed.ncbi.nlm.nih.gov/14404178/
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A novel 39-plex typing system for single nucleotide polymorphisms (SNPs) has been developed. This multiplex approach has the advantage of being able to type 38 autosomal SNPs and one sex-discriminating base exchange site on the X and Y chromosomes rapidly and simultaneously. The SNP loci on the autosomes, which we examined, contain 15 loci distributed on blood type genes: three on RhCE, two each on Km and Gc, and one each on Duffy, AcP1, Tf, MN, GPT, EsD, PI, and Kidd genes. Thirty-seven genomic DNA fragments containing a total of 38 SNPs and one sex-discriminating site were amplified in one multiplex PCR reaction. Following the reaction, single nucleotide primer extension reaction was performed by dividing these SNP loci into five groups. The SNP type of each of the 39 loci was determined at one time by capillary electrophoresis using the newly designed multi-injection method. The combined PD (power of discrimination) of this typing system was (1-1.1) x 10(-14), and the MEC (mean exclusion chance) was 0.9990. We applied this system to forensic cases, including 16 paternity testing cases (13 non-exclusion and three exclusion cases) and one personal identification case. For the paternity testing cases, the highest Essen-Möller's W-value was 0.9999995. The pM (matching probability) of the personal identification case was 2.22 x 10(-17). These data showed that this system was an excellent tool for use in forensic cases of paternity testing and personal identification.
We aimed to evaluate whether a PEG-IAT method, modified for gel technique, could replace the two-stage enzyme method for routine identification of red blood cell (RBC) antibodies.A total of 161 selected patient samples were analyzed with the two-stage enzyme tube technique and the PEG-IAT in gel techniques. In 65 samples, identical antibody results were obtained by the two methods. An additional 28 antibodies were identified by either the PEG-IAT gel method or the enzyme method. Twenty antibodies were identified by the PEG-IAT gel method only (12 Rh antibodies, four anti-Kell, two anti-Fya, two anti-Jka), and eight antibodies by the enzyme method only (four Rh antibodies, one anti-K, one anti-Jka, two anti-Lea). The results in the remaining 68 samples were judged as nonspecific reactions, which were more common when the enzyme method was used. We concluded that the PEG-IAT in gel method identifies more RBC antibodies with less nonspecific reactions than the two-stage enzyme technique. However, no single method detected all clinical significant RBC antibodies.
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