T cells which proliferate in response to concanavalin A include cells which proliferate in mixed leucocyte reactions.

T Watanabe; C G Fathman; A Coutinho

doi:10.1002/eji.1830070905

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T cells which proliferate in response to concanavalin A include cells which proliferate in mixed leucocyte reactions.

T Watanabe·C G Fathman·A Coutinho

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Abstract

Selection in long-term culture of alloreactive T cells, by successive in vitro restimulation with semi-allogeneic cells, results in primed responder cell populations which maintain full proliferative reactivity to allogeneic cells as well as to the T cell mitogens concanavalin A (Con A) and phytohemagglutinin (PHA) but are depleted of cells which can effect target cell destruction in either a specific or nonspecific manner. Con A-induced T cell blasts (selected by velocity sedimentation) can revert to small resting lymphocytes in the presence of inert "filler" cells. Con A blasts which have reverted, readily proliferate in response to Con A or allogeneic stimulator cells but are largely depleted of effector killer cells and PHA-responsive cells.

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Animals Cells, Cultured Concanavalin A In Vitro Techniques Killer Cells, Natural Lymphocyte Activation Lymphocyte Cooperation Lymphocyte Culture Test, Mixed Mice Mice, Inbred Strains Spleen T-Lymphocytes Journal Article

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T Watanabe, C G Fathman, A Coutinho. 1977. T cells which proliferate in response to concanavalin A include cells which proliferate in mixed leucocyte reactions.. https://doi.org/10.1002/eji.1830070905

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