Sequence accuracy of large DNA sequencing projects.

W Q Chen; T Hunkapiller

doi:10.3109/10425179209020814

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PubMed · 1446072

Sequence accuracy of large DNA sequencing projects.

W Q Chen·T Hunkapiller

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Abstract

Very little information has been accumulated regarding the likely accuracy of final or consensus DNA sequence data. With the large-scale efforts anticipated for the Human Genome Project, the subjective determination of final sequence must eventually be replaced with more objective, automatic methods. This will require a much better understanding of the nature of error in raw sequencing data and its impact on the determination of the final sequence. This paper describes a start at defining the error model of large-scale sequencing efforts based on random subcloning strategies.

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Consensus Sequence Models, Statistical Reproducibility of Results Sequence Analysis, DNA Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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W Q Chen, T Hunkapiller. 1992. Sequence accuracy of large DNA sequencing projects.. https://doi.org/10.3109/10425179209020814

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