A rat brain-derived neurotrophic factor-encoding gene generates multiple transcripts through alternative use of 5' exons and polyadenylation sites.

O Ohara; Y Gahara; H Teraoka; T Kitamura

doi:10.1016/0378-1119(92)90148-i

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A rat brain-derived neurotrophic factor-encoding gene generates multiple transcripts through alternative use of 5' exons and polyadenylation sites.

O Ohara·Y Gahara·H Teraoka·T Kitamura

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Abstract

As a first step toward clarification of the transcriptional controls of the gene encoding brain-derived neurotrophic factor (BDNF), we cloned and sequenced a rat genomic DNA fragment carrying this gene. RNA blotting analysis using a probe derived from the 3'-flanking region of BDNF revealed that alternative use of 3'-polyadenylation sites generates at least two BDNF transcripts that differ in the size of the 3'-noncoding region. Furthermore, sequence analysis of the 5'-end of the BDNF cDNA revealed the presence of at least six different types of transcripts which were probably derived through alternative use of the multiple 5'-exons. Therefore, a single BDNF gene could produce multiple types of transcripts with different noncoding sequences through alternative use of both 5'-exons and 3'-transcription termination sites.

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Alternative Splicing Animals Base Sequence Brain-Derived Neurotrophic Factor Cloning, Molecular DNA Exons Molecular Sequence Data Nerve Growth Factors Nerve Tissue Proteins Poly A RNA Processing, Post-Transcriptional RNA, Messenger Rats Restriction Mapping Journal Article

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O Ohara, Y Gahara, H Teraoka, T Kitamura. 1992-11-16. A rat brain-derived neurotrophic factor-encoding gene generates multiple transcripts through alternative use of 5' exons and polyadenylation sites.. https://doi.org/10.1016/0378-1119(92)90148-i

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