[Flow karyotyping and chromosome sorting].

Abstract

For flow karyotyping and sorting, chromosomes are stained in suspension with appropriate DNA fluorochromes, and passed singly through a beam of laser light. The emitted pulses of fluorescence and scattered light are measured and stored in the form of a histogram displaying the number of pulses versus fluorescence or scattered light intensity. A data processing system was devised to remove signals from cell debris or chromosome fragments. A selective window was set in a dot plot of fluorescence versus scattered light intensities and a program was made to count pulses from the gated area only. As application of chromosome fractionation, flow cytometry has been used for karyotype analysis, gene mapping, and chromosomal DNA library construction.