Structural basis for the E1/E1P-E2/E2P conformation changes in the sarcoplasmic reticulum Ca(2+)-ATPase studied by site-specific mutagenesis.

J P Andersen; B Vilsen

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PubMed · 1449062

Structural basis for the E1/E1P-E2/E2P conformation changes in the sarcoplasmic reticulum Ca(2+)-ATPase studied by site-specific mutagenesis.

J P Andersen·B Vilsen

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Abstract

Site-specific mutagenesis and functional expression of cloned cDNA of the sarcoplasmic reticulum Ca(2+)-ATPase have been used to demonstrate that exchange of single amino acid residues in the Ca(2+)-ATPase can lead to forms with either "E1/E1P" or "E2/E2P" characteristics. The localization of the mutated residues identifies the M4S4 segment of the ATPase molecule as central to the energy-tranducing conformation change, and on the basis of this information a model for the pump mechanism is discussed.

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Animals Calcium-Transporting ATPases DNA Models, Chemical Muscles Mutagenesis Phosphorylation Protein Conformation Rabbits Sarcoplasmic Reticulum Journal Article Research Support, Non-U.S. Gov't

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J P Andersen, B Vilsen. 1992. Structural basis for the E1/E1P-E2/E2P conformation changes in the sarcoplasmic reticulum Ca(2+)-ATPase studied by site-specific mutagenesis.. https://pubmed.ncbi.nlm.nih.gov/1449062/

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