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PubMed · 14501071

Virus-induced gene silencing.

S P Dinesh-Kumar·Radhamani Anandalakshmi·Rajendra Marathe·Michael Schiff·Yule Liu
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Abstract

In the postgenomic era, large-scale functional genomic approaches are necessary for converting sequence information into functional information. A para-genetic approach, called virus-induced gene silencing (VIGS), offers a rapid means of gaining insight into gene function in plants. VIGS system could be used to suppress endogenous gene expression by infecting plants with a recombinant virus vector (VIGS vector) carrying host-derived sequence. Here, we describe the use of tobacco rattle virus (TRV)-based VIGS technique to study gene function in Nicotiana benthamiana and tomato.

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Gene SilencingGenetic TechniquesGenetic VectorsSolanum lycopersicumPlant LeavesRhizobiumNicotianaTobacco Mosaic VirusJournal ArticleResearch Support, Non-U.S. Gov't

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S P Dinesh-Kumar, Radhamani Anandalakshmi, Rajendra Marathe, Michael Schiff, Yule Liu. 2003. Virus-induced gene silencing.. https://doi.org/10.1385/1-59259-413-1%3A287

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