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A simple, low-cost device for processing and embedding tissues at sub-zero temperatures.

Wayne Shoemaker·Craig Hayes·Thomas E Phillips
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Abstract

A simple apparatus to maintain tissues samples at sub-zero temperatures during dehydration, infiltration, and polymerization is described. The device uses a conventional siphon-type carbon dioxide gas cylinder to maintain an aluminum block at temperatures as low as -35 degrees C for over 15 hours/cylinder.

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AnimalsCold TemperatureFreeze DryingFreezingHistological TechniquesIleumMiceTemperatureTissue EmbeddingJournal Article

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Wayne Shoemaker, Craig Hayes, Thomas E Phillips. 2003-10-15. A simple, low-cost device for processing and embedding tissues at sub-zero temperatures.. https://doi.org/10.1002/jemt.10390

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