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Truncated pre-S/S proteins transactivate multiple target sequences.

G Natoli·C Balsano·M L Avantaggiati·E De Marzio·M Artini·D Collepardo·E Elfassi·M Levrero
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Abstract

In order to investigate the transactivational function of HBV truncated preS/S proteins we have constructed two sets of plasmids and have tested their transactivational potential on the c-myc regulatory sequences and the TPA-responsive element. We found that preS/S proteins only become transactivationally active when truncated at the carboxy terminal end. Furthermore, using immunofluorescence microscopy we determined that the proteins are located exclusively in the cytoplasm, apparently ruling out DNA binding and activation of factors in the nucleus.

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Chloramphenicol O-AcetyltransferaseDNA Mutational AnalysisGenes, mycHepatitis B Surface AntigensHepatitis B virusProtein PrecursorsRecombinant Fusion ProteinsRegulatory Sequences, Nucleic AcidStructure-Activity RelationshipTranscriptional ActivationComparative StudyJournal Article

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G Natoli, C Balsano, M L Avantaggiati, E De Marzio, M Artini, D Collepardo, E Elfassi, M Levrero. 1992. Truncated pre-S/S proteins transactivate multiple target sequences.. https://doi.org/10.1007/978-3-7091-5633-9_14

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