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Conjugate DNAzymes.

Takanori Kubo·Kengo Takamori·Rumiana Bakalova·Hideki Ohba·Masayuki Fujii
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Abstract

Conjugate DNAzymes were synthesized by solid phase fragment condensation and their biological properties were characterized. They have increased affinity to target RNA, enhanced stability against DNase 1 digestion and comparable or higher RNA cleaving activity compared with native and also phosphorothioate DNA zymes. It was also demonstrated that conjugate DNAzymes could inhibit BCR-ABL tyrosine kinase in cellular lysis of human leukemia cell line. Consequently DNAzymes can be expected to act effectively in cellular system and also in vivo system.

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Base SequenceDNA, CatalyticFusion Proteins, bcr-ablHumansLeukemiaPeptidesProtein-Tyrosine KinasesTumor Cells, CulturedJournal Article

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Takanori Kubo, Kengo Takamori, Rumiana Bakalova, Hideki Ohba, Masayuki Fujii. 2003. Conjugate DNAzymes.. https://doi.org/10.1093/nass%2F3.1.177

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