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Polycation-accelerated strand exchange (PASE) for SNPs typing.

Won Jong Kim·Yuichi Sato·Toshihiro Akaike·Atsushi Maruyama
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Abstract

In the previous study (Chem. Eur. J., 7, 176 (2001), J. Am. Chem. Soc., 124, 12676 (2002)), we demonstrated that the cationic comb-type copolymer (PLL-g-Dex, CCC) composed of a cationic poly(L-lysine) backbone and water-soluble side chains of dextran accelerated the DNA strand exchange reaction between double helical DNA and its homologous single strand. In this study, Polycation-Accelerated Strand Exchange (PASE) assay was applied to discriminate single-base mismatch in DNA sequence such as single nucleotide polymorphisms (SNPs). We could discriminate the 20mer DNA with a single-base mismatch from the full matched DNA with high efficacy by PASE.

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Base Pair MismatchBase SequenceDNAPolyaminesPolyelectrolytesPolymorphism, Single NucleotideJournal Article

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Won Jong Kim, Yuichi Sato, Toshihiro Akaike, Atsushi Maruyama. 2003. Polycation-accelerated strand exchange (PASE) for SNPs typing.. https://doi.org/10.1093/nass%2F3.1.273

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