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PubMed · 14511252

Decrease of deleted in malignant brain tumour-1 (DMBT-1) expression is a crucial late event in intrahepatic cholangiocarcinoma.

M Sasaki·S-F Huang·M-F Chen·Y-Y Jan·T-S Yeh·A Ishikawa·J Mollenhauer·A Poustka·K Tsuneyama·Y Nimura·K Oda·Y Nakanuma
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Abstract

AIMS: To investigate the participation of DMBT-1, a candidate tumour suppressor gene, in the development of intrahepatic cholangiocarcinoma via intraductal papillary neoplasm of the liver (IPN-L) arising in hepatolithiasis. DMBT-1 plays a role in mucosal immune defence. METHODS AND RESULTS: The expression of DMBT-1 was examined immunohistochemically in biliary epithelial cells in hepatolithiasis (n = 25), invasive and non-invasive cholangiocarcinoma associated with hepatolithiasis (n = 52), IPN-L with hepatolithiasis (n = 49), cholangiocarcinoma without hepatolithiasis (n = 32), and 10 normal control livers. DMBT-1 was expressed more frequently in the biliary epithelia of hepatolithiasis when compared with normal livers (P < 0.05). DMBT-1 expression was also frequent in IPN-L (57%) and non-invasive cholangiocarcinoma (79%). By contrast, DMBT-1 was decreased in invasive cholangiocarcinoma with and without hepatolithiasis (50% and 30%, respectively) (P < 0.05). The homozygous deletion of the DMBT-1 gene was recognized in four (20%) of 20 cholangiocarcinoma tissues and two (50%) of four cholangiocarcinoma cell lines, corresponding to the reduction of DMBT-1 expression. No deletion was detected in hepatolithiasis tissues. CONCLUSION: DMBT-1 expression is increased in IPN-L and non-invasive cholangiocarcinoma as well as in biliary epithelia in hepatolithiasis. Decreased expression of DMBT-1 and homozygous deletion of the DMBT-1 gene in invasive cholangiocarcinoma suggest that they occur in the late stage of cholangiocarcinogenesis.

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AgglutininsBile Duct NeoplasmsBile Ducts, IntrahepaticBrain NeoplasmsCalcium-Binding ProteinsCarcinoma, PapillaryCell Line, TumorCholangiocarcinomaDNA-Binding ProteinsGene DeletionGene Expression Regulation, NeoplasticHumansImmunoenzyme TechniquesIn Situ HybridizationLithiasisRNA, MessengerRNA, NeoplasmReceptors, Cell SurfaceReverse Transcriptase Polymerase Chain ReactionTumor Suppressor ProteinsJournal Article

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M Sasaki, S-F Huang, M-F Chen, Y-Y Jan, T-S Yeh, A Ishikawa, J Mollenhauer, A Poustka, K Tsuneyama, Y Nimura, K Oda, Y Nakanuma. 2003. Decrease of deleted in malignant brain tumour-1 (DMBT-1) expression is a crucial late event in intrahepatic cholangiocarcinoma.. https://doi.org/10.1046/j.1365-2559.2003.01719.x

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