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PubMed · 14513796

High throughput screening for intercellular adhesion molecule-1 inhibitor.

De-cheng Ren·Guan-hua Du·Jun-tian Zhang
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Abstract

AIM: To develop a high throughput screening assay to identify inhibitors of intercellular adhesion molecule-1 (ICAM-1) expression in human umbilical vein endothelial cells (HUVEC). METHODS: ICAM-1 expression in lipopolysaccharide-stimulated endothelial cells was measured by ELISA. The cytotoxicity of the compounds was measured by MTT. RESULTS: Lipopolysaccharide (LPS) increased ICAM-1 expression in HUVEC in a concentration- and time-dependent manner. Two thousand compounds were screened and the hit rate was 1.5%. Among these 30 compounds, 24 were cytotoxic. CONCLUSION: The ELISA method was inexpensive, reproducible and suitable for high throughput primary cell assay. This assay was feasible to identify inhibitors of ICAM-1 and simultaneously discriminate the activity from the cytotoxic effects.

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Cell Adhesion MoleculesCells, CulturedChemistry, PharmaceuticalDrug Evaluation, PreclinicalEndothelium, VascularHumansIntercellular Adhesion Molecule-1LipopolysaccharidesUmbilical VeinsJournal ArticleResearch Support, Non-U.S. Gov't

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De-cheng Ren, Guan-hua Du, Jun-tian Zhang. 2003. High throughput screening for intercellular adhesion molecule-1 inhibitor.. https://pubmed.ncbi.nlm.nih.gov/14513796/

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