Drop-delay measurement using enzyme-coated particles.

Y A Lazebnik; A I Poletaev; V V Zenin

doi:10.1002/cyto.990130614

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Drop-delay measurement using enzyme-coated particles.

Y A Lazebnik·A I Poletaev·V V Zenin

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Abstract

A simple technique is suggested for the measurement of drop delay for flow sorting. While the flow cytometer was set to sort a fixed number of particles, the drop-delay setting was changed step by step, and at each step the HRP-coupled particles were sorted into a well of an immunoassay strip. Then the HRP activity of the sorted samples was revealed by routine methods. The maximum level of the enzyme activity shows the proper drop-delay setting. Determination of the drop-delay setting takes only a few minutes. The technique is independent of the type of flow cytometer and does not require any additional equipment.

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Y A Lazebnik, A I Poletaev, V V Zenin. 1992. Drop-delay measurement using enzyme-coated particles.. https://doi.org/10.1002/cyto.990130614

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