Dicer is essential for mouse development.

Emily Bernstein; Sang Yong Kim; Michelle A Carmell; Elizabeth P Murchison; Heather Alcorn; Mamie Z Li; Alea A Mills; Stephen J Elledge; Kathryn V Anderson; Gregory J Hannon

doi:10.1038/ng1253

PubMed · 14528307

Dicer is essential for mouse development.

Emily Bernstein·Sang Yong Kim·Michelle A Carmell·Elizabeth P Murchison·Heather Alcorn·Mamie Z Li·Alea A Mills·Stephen J Elledge·Kathryn V Anderson·Gregory J Hannon

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Abstract

To address the biological function of RNA interference (RNAi)-related pathways in mammals, we disrupted the gene Dicer1 in mice. Loss of Dicer1 lead to lethality early in development, with Dicer1-null embryos depleted of stem cells. Coupled with our inability to generate viable Dicer1-null embryonic stem (ES) cells, this suggests a role for Dicer, and, by implication, the RNAi machinery, in maintaining the stem cell population during early mouse development.

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Amino Acid Sequence Animals DEAD-box RNA Helicases Embryonic and Fetal Development Endoribonucleases Mice Mice, Knockout Molecular Sequence Data RNA Helicases RNA Interference Ribonuclease III Stem Cells Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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Emily Bernstein, Sang Yong Kim, Michelle A Carmell, Elizabeth P Murchison, Heather Alcorn, Mamie Z Li, Alea A Mills, Stephen J Elledge, Kathryn V Anderson, Gregory J Hannon. 2003-10-05. Dicer is essential for mouse development.. https://doi.org/10.1038/ng1253

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