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PubMed · 14551936

[Endothelial cell proliferation stimulated by basic fibroblast growth factor].

Wei-li Lu·Yu-lan Dong
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Abstract

OBJECTIVE: To determine the influence of basic fibroblast growth factor (bFGF) on endothelial cell (EC) proliferation in vitro and its possible mechanisms, and to examine the effect of both TNP-470 and dexamethasone (Dex) on the EC proliferation induced by bFGF. METHODS: Human umbilical vein endothelial cells were cultured and the proliferation of EC was quantified by a colorimetric assay using MTT reagent. The expression of nuclear factor-kappa B (NF-kappa B) and ki-67 was detected with SABC immunohistochemical method. RESULTS: bFGF stimulated the EC proliferation and enhanced the expression of NF-kappa B and ki-67 in nucleus; TNP-470 and Dex suppressed EC proliferation induced by bFGF, and reduced the expression of NF-kappa B and ki-67 in nucleus. CONCLUSION: The above results indicate that the possible mechanisms of EC proliferation stimulated by bFGF come from that bFGF can activate NF-kappa B to promote the synthesis of DNA and EC mitosis. TNP-470 and Dex inhibited EC proliferation stimulated by bFGF by inhibiting NF-kappa B.

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Cell DivisionCell NucleusCells, CulturedDNADexamethasoneEndothelium, VascularFibroblast Growth Factor 2HumansKi-67 AntigenNF-kappa BTumor Necrosis Factor-alphaUmbilical VeinsEnglish AbstractJournal ArticleResearch Support, Non-U.S. Gov't

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Wei-li Lu, Yu-lan Dong. 2003. [Endothelial cell proliferation stimulated by basic fibroblast growth factor].. https://pubmed.ncbi.nlm.nih.gov/14551936/

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