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PubMed · 14558996

Microplate based biosensing with a computer screen aided technique.

Daniel Filippini·Tony P M Andersson·Samuel P S Svensson·Ingemar Lundström
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Abstract

Melanophores, dark pigment cells from the frog Xenopus laevis, have the ability to change light absorbance upon stimulation by different biological agents. Hormone exposure (e.g. melatonin or alpha-melanocyte stimulating hormone) has been used here as a reversible stimulus to test a new compact microplate reading platform. As an application, the detection of the asthma drug formoterol in blood plasma samples is demonstrated. The present system utilizes a computer screen as a (programmable) large area light source, and a standard web camera as recording media enabling even kinetic microplate reading with a versatile and broadly available platform, which suffices to evaluate numerous bioassays. Especially in the context of point of care testing or self testing applications these possibilities become advantageous compared with highly dedicated comparatively expensive commercial systems.

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AnimalsBiological AssayBiosensing TechniquesCell AggregationCell CountCell Culture TechniquesCell MovementCells, CulturedComputer TerminalsDose-Response Relationship, DrugEquipment DesignEquipment Failure AnalysisEthanolaminesFormoterol FumarateImage Interpretation, Computer-AssistedMelanophoresMelatoninOnline SystemsPhotomicrographyXenopus laevisComparative StudyEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov'tValidation Study

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Daniel Filippini, Tony P M Andersson, Samuel P S Svensson, Ingemar Lundström. 2003-10-30. Microplate based biosensing with a computer screen aided technique.. https://doi.org/10.1016/s0956-5663(03)00132-5

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