Total stepwise solid-phase peptide-oligonucleotide conjugate synthesis on macroporous polystyrene.

Dmitry A Stetsenko; Andrey D Malakhov; Michael J Gait

doi:10.1081/ncn-120022970

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Total stepwise solid-phase peptide-oligonucleotide conjugate synthesis on macroporous polystyrene.

Dmitry A Stetsenko·Andrey D Malakhov·Michael J Gait

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Abstract

An efficient total stepwise solid-phase synthesis of oligonucleotide-peptide conjugates on a macroporous polystyrene is described. Extending our homoserine linker approach, we prepared a range of fluorescein-labelled conjugates containing one of two different peptides together with oligonucleotides containing 2'-deoxynucleoside or 2'-O-methylribonucleoside phosphodiesters, or gapmers containing 2'-deoxyphosphorothioate sequences flanked by 2'-O-methyl wings.

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Amino Acid Sequence Base Sequence Fluorescein Molecular Sequence Data Oligodeoxyribonucleotides Oligodeoxyribonucleotides, Antisense Oligopeptides Peptide Nucleic Acids Polystyrenes Journal Article Research Support, Non-U.S. Gov't

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Dmitry A Stetsenko, Andrey D Malakhov, Michael J Gait. Total stepwise solid-phase peptide-oligonucleotide conjugate synthesis on macroporous polystyrene.. https://doi.org/10.1081/ncn-120022970

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