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Endcaps for stabilizing short DNA duplexes.

Pei-Sze Ng·Maneesh R Pingle·Ganesan Balasundarum·Alan Friedman·Xiaolin Zu·Donald E Bergstrom
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Abstract

The syntheses of endcaps for covalently linking the 3' and 5' hydroxyl groups of blunt end double-stranded DNA are described. Endcap diols were converted into DMTr protected phosphoramidites and incorporated between nucleotides 4 and 5 of a self-complementary octamer. The stabilizing effect of the endcaps on duplex DNA was determined by Tm experiments on the self-complementary octamer.

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Base SequenceDNADrug StabilityModels, MolecularNucleic Acid ConformationNucleic Acid DenaturationThermodynamicsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Pei-Sze Ng, Maneesh R Pingle, Ganesan Balasundarum, Alan Friedman, Xiaolin Zu, Donald E Bergstrom. Endcaps for stabilizing short DNA duplexes.. https://doi.org/10.1081/ncn-120023088

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