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PNA-DNA chimeras forming quadruplex structures.

Veronica Esposito·Aldo Galeone·Luciano Mayol·Anna Messere·Gennaro Piccialli·Antonio Randazzo
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Abstract

1H-NMR, CD, and UV spectroscopy have been used to investigate the structure of PNA/DNA chimeras forming quadruplex structures. In particular, we synthesized 5'TGGG3'-t (1) and 5'TGG3'-gt (2), where lower and upper case letters indicate PNA and DNA residues, respectively. CD spectrum and all NMR data of (1) are typical of quadruplexes involving four parallel strands. UV melting profile of (1) indicates that its thermal stability is quite similar to that observed for the reference structure [d(TGGGT)]4. 1H-NMR spectrum for 5'TGG3'-gt (2) shows that this oligonucleotide is not able to fold into a single, well-defined species.

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Base SequenceChimeraDNAMagnetic Resonance SpectroscopyNucleic Acid ConformationOligodeoxyribonucleotidesPeptide Nucleic AcidsJournal ArticleResearch Support, Non-U.S. Gov't

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Veronica Esposito, Aldo Galeone, Luciano Mayol, Anna Messere, Gennaro Piccialli, Antonio Randazzo. PNA-DNA chimeras forming quadruplex structures.. https://doi.org/10.1081/ncn-120023112

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