PubMed Health⌕ Search

PubMed · 14565856

Interdisciplinary research: putting the methods under the microscope.

Abstract

BACKGROUND: While the desirability of interdisciplinary inquiry has been widely acknowledged, indeed has become 'the mantra of science policy', the methods of interdisciplinary collaboration are opaque to outsiders and generally remain undescribed. DISCUSSION: Many have analysed interdisciplinarity, especially in relation to the creation of new disciplines and institutions. These analyses are briefly outlined. Still, there currently persists a silence about the methods of interdisciplinary collaboration itself, and the core of this paper proposes a template for such methods. SUMMARY: Breaking this silence--by making the methods of interdisciplinary projects transparent--could further invigorate interdisciplinary research.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

David W Robertson, Douglas K Martin, Peter A Singer. 2003-10-19. Interdisciplinary research: putting the methods under the microscope.. https://doi.org/10.1186/1471-2288-3-20

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

Compound exocytosis of secretory granules containing salivary chromogranin A in granular duct cells in rat submandibular gland: the last study in collaboration with the late Professor Noboru Yanaihara at Yanaihara Institute.

Chromogranin A (CgA) is a member of a family of chromogranins, which are co-stored and co-released with adrenaline and noradrenalin (NAd) in the adrenal medulla in response to stimulation of the splanchnic nerve. Double immunohistochemical staining is carried out by use of antibodies against CgA and 1,4,5-trisphosphate receptor type 2 (IP3R2) on the same sections prepared from the isolated and perfused submandibulllar gland of rat. In the control sections prepared from resting state, an intense IP3R2 immunoreactivity (IR) appeared preferentially at the apical pole of subpopulation of the granulated duct cells, in which CgA-like IR distributed throughout the cytoplasm. Electron-micrograph showed that the granular cells in the resting state stored numerous membrane-bound granules in the apical cytoplasm. Stimulation with 1 microM NAd caused rapid immediate increase in secretory responses. Sections prepared from the gland at the peak of secretory responses exhibited that, in the granular duct cells, the apically converge IP3R2 IR became diffuse and indistinguishable, and that the apical half of the cells was occupied, indicating that mobilization of Ca2+ from the IP3-sensitive pool may preferentially be involved in the secretory responses to alpha-adrenergic agonist.

Academies and Institutes↗