[A simple and highly efficient Agrobacterium-mediated rice transformation system].

Mei Ru Li; Hong Qing Li

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PubMed · 14574993

[A simple and highly efficient Agrobacterium-mediated rice transformation system].

Mei Ru Li·Hong Qing Li

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Abstract

A simple and highly efficient rice transformation system was established based on the studying of factors influencing the Agrobacterium-mediated rice transformation. Embryogenic calli derived from mature embryos were infected and cocultivated with A. tumefaciens EHA101 harboring binary vectors: pHQ9, pHQ10, pHQT3. The highest transformation frequency was about 100 hygromycin resistance calli per gram of calli explants, and above 85% of these calli could be regenerated into plants. The putative transgenic plants were confirmed by GUS assay and southern-blot analysis. Hygromycin resistance tests indicated that the segregation of transgene in T1 progeny corresponded to the Mendelian ratio, 3:1. This system will benefit the functional genomic study of rice by using T-DNA insertion mutagenesis and gene targeting.

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Agrobacterium tumefaciens Cinnamates Drug Resistance Genetic Vectors Hygromycin B Oryza Plants, Genetically Modified Promoter Regions, Genetic Transformation, Genetic Journal Article Research Support, Non-U.S. Gov't

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Mei Ru Li, Hong Qing Li. 2003. [A simple and highly efficient Agrobacterium-mediated rice transformation system].. https://pubmed.ncbi.nlm.nih.gov/14574993/

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