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Analysis of protein interactions using fluorescence technologies.

Yuling Yan·Gerard Marriott
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Abstract

Biophotonics techniques, especially those involving fluorescence, are widely used in proteomics to characterize the in vitro interactions between proteins in high-throughput mode. On the other hand, fluorescence-based imaging studies often show that protein activity is regulated through large protein complexes that transiently form at specific sites in the cell. One could therefore argue that a systematic functional analysis of the human proteome requires technologies that are capable of time and spatially resolved, multiplexed analysis of protein interactions within cells.

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Fluorescence Resonance Energy TransferFluorescent DyesMicroscopy, FluorescenceProtein Interaction MappingProteinsProteomeJournal ArticleResearch Support, U.S. Gov't, P.H.S.Review

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Yuling Yan, Gerard Marriott. 2003. Analysis of protein interactions using fluorescence technologies.. https://doi.org/10.1016/j.cbpa.2003.08.017

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