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PubMed · 14584692

Improved spiral disk assembly for high-speed counter-current chromatography.

Yoichiro Ito·Fuquan Yang·Paul Fitze·Jimmie Powell·David Ide
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Abstract

The spiral disk for type-J high-speed counter-current chromatography (HSCCC) has been improved by placing short segments of PTFE (tetrafluoroethylene) tubing into the channel at regular intervals. The best results were obtained with a four-channel spiral column using 600 spacers, which significantly improved stationary phase retention and partition efficiency in both n-butanol-acetic acid-water (4:1:5) and poly(ethylene glycol) 1000-dibasic phosphate polymer phase systems. Based on these findings, a "bead-chain spiral disk" was designed and its performance tested with a type-J HSCCC centrifuge. This new column design substantially improved the separation of both dipeptide and protein samples in terms of stationary phase retention and partition efficiency.

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Yoichiro Ito, Fuquan Yang, Paul Fitze, Jimmie Powell, David Ide. 2003-10-31. Improved spiral disk assembly for high-speed counter-current chromatography.. https://doi.org/10.1016/j.chroma.2003.08.011

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