Virus particle detection by solid phase immunocapture and atomic force microscopy.

Saju R Nettikadan; James C Johnson; Curtis Mosher; Eric Henderson

doi:10.1016/j.bbrc.2003.10.022

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Virus particle detection by solid phase immunocapture and atomic force microscopy.

Saju R Nettikadan·James C Johnson·Curtis Mosher·Eric Henderson

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Abstract

A novel application of atomic force microscopy (AFM) in the rapid, label-free detection and identification of viruses is described. Multiplexed, miniaturized antibody domains were constructed using "ink-jet" protein arraying technology. The solid-phase affinity substrate termed the "ViriChip" was used in the immunocapture of bacteriophage fd, canine parvoviruses, and coxsackieviruses and analyzed by AFM. Immunocapture was found to be antibody-specific with a sensitivity of 10(8)pfu/ml in 30min. Virus binding was found to be linear for concentration between 10(8) and 10(10)pfu/ml and did not reach saturation through 4h.

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Equipment Design Equipment Failure Analysis Immunoassay Microscopy, Atomic Force Protein Array Analysis Reproducibility of Results Sensitivity and Specificity Viruses Comparative Study Evaluation Study Journal Article Research Support, U.S. Gov't, P.H.S.Validation Study

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Saju R Nettikadan, James C Johnson, Curtis Mosher, Eric Henderson. 2003-11-14. Virus particle detection by solid phase immunocapture and atomic force microscopy.. https://doi.org/10.1016/j.bbrc.2003.10.022

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