pHP489, a Helicobacter pylori small cryptic plasmid, harbors a novel gene coding for a replication initiation protein.

Jae-Young Song; Seong-Gyu Park; Hyung-Lyun Kang; Woo-Kon Lee; Myung-Je Cho; Jeong-Uck Park; Seung-Chul Baik; Hee-Shang Youn; Gyung-Hyuck Ko; Kwang-Ho Rhee

doi:10.1016/s0147-619x(03)00061-1

PubMed · 14597012

pHP489, a Helicobacter pylori small cryptic plasmid, harbors a novel gene coding for a replication initiation protein.

Jae-Young Song·Seong-Gyu Park·Hyung-Lyun Kang·Woo-Kon Lee·Myung-Je Cho·Jeong-Uck Park·Seung-Chul Baik·Hee-Shang Youn·Gyung-Hyuck Ko·Kwang-Ho Rhee

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Abstract

We have analyzed a Helicobacter pylori plasmid, pHP489. The 1222-bp nucleotide sequence had one open reading frame, a DnaA-binding site, one direct repeat, and three inverted repeats. The (G+C) content of pHP489 was 33.3%. Although the nucleic acid sequence and deduced amino acid sequence were homologous to those of other bacterial plasmid Rep proteins, the degree of similarity was very low. A deletion analysis showed that the Rep protein was not required for the replication of pHP489 in its H. pylori host, but the host replication machinery was needed.

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Amino Acid Sequence Bacterial Proteins Base Composition Base Sequence Binding Sites DNA Replication DNA-Binding Proteins Helicobacter pylori Molecular Sequence Data Plasmids Sequence Homology, Amino Acid Journal Article Research Support, Non-U.S. Gov't

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Jae-Young Song, Seong-Gyu Park, Hyung-Lyun Kang, Woo-Kon Lee, Myung-Je Cho, Jeong-Uck Park, Seung-Chul Baik, Hee-Shang Youn, Gyung-Hyuck Ko, Kwang-Ho Rhee. 2003. pHP489, a Helicobacter pylori small cryptic plasmid, harbors a novel gene coding for a replication initiation protein.. https://doi.org/10.1016/s0147-619x(03)00061-1

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