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Identification of estrogen-responsive genes by complementary deoxyribonucleic acid microarray and characterization of a novel early estrogen-induced gene: EEIG1.

Dong-Yu Wang·Roberta Fulthorpe·Steven N Liss·Elizabeth A Edwards
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Abstract

Estrogen receptors (ERs) are nuclear transcription factors that regulate gene expression in response to estrogen and estrogen-like compounds. Identification of estrogen-regulated genes in target cells is an essential step toward understanding the molecular mechanisms of estrogen action. Using cDNA microarray examinations, 19 genes were identified as induced by 17 beta-estradiol in MCF-7 cells, 10 of which have been reported previously to be estrogen responsive or to be linked with ER status. Five known estrogen-regulated genes, E2IG4, IGFBP4, SLC2A1, XBP1 and B4GALT1, and AFG3L1, responded quickly to estrogen treatment. A novel estrogen-responsive gene was identified and named EEIG1for early estrogen-induced gene 1. EEIG1 was clearly induced by 17 beta-estradiol within 2 h of treatment, and was widely responsive to a group of estrogenic compounds including natural and synthetic estrogens and estrogenic environmental compounds. EEIG1 was expressed in ER-positive but not in ER-negative breast cancer cell lines. EEIG1 expression was repressed by antiestrogens 4-OH-tamoxifen and ICI 182,780 but not by protein synthesis inhibitors cycloheximide and puromycin. These results provide evidence that some estrogenic compounds differentially enhance the transcription of estrogen-regulated genes and suggest a role for EEIG1 in estrogen action.

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Breast NeoplasmsCycloheximideDNA-Binding ProteinsDose-Response Relationship, DrugEstradiolEstrogen AntagonistsEstrogensFemaleFulvestrantGalactosyltransferasesGene Expression RegulationHumansInsulin-Like Growth Factor Binding Protein 4Neoplasm ProteinsNuclear ProteinsOligonucleotide Array Sequence AnalysisPesticidesPhytoestrogensProtein Synthesis InhibitorsPuromycinReceptors, EstrogenRegulatory Factor X Transcription FactorsTamoxifenTranscription FactorsTumor Cells, CulturedX-Box Binding Protein 1Journal ArticleResearch Support, Non-U.S. Gov't

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Dong-Yu Wang, Roberta Fulthorpe, Steven N Liss, Elizabeth A Edwards. 2003-11-06. Identification of estrogen-responsive genes by complementary deoxyribonucleic acid microarray and characterization of a novel early estrogen-induced gene: EEIG1.. https://doi.org/10.1210/me.2003-0202

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