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Microdevice for separation and quantitative fraction collection.

Michal Spesný·Frantisek Foret
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Abstract

A new microfluidic concept for quantitative whole-column fraction collection of electrophoretically separated zones was developed. The prototype device, fabricated on a polycarbonate disk by injection molding, integrated electrophoretic separation channels with fraction collection reservoirs distributed along the separation channel. The microdevice was designed in a CD-like format to use the centrifugal force for moving the liquid in the microchannels. A serpentine shape of the separation channel was selected to create segments for quantitative whole-column fraction collection. The operation was tested with visual monitoring of isotachophoretic separation and collection of cationic dyes.

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DNAMicrofluidicsMiniaturizationPeptidesProteinsJournal ArticleResearch Support, Non-U.S. Gov't

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Michal Spesný, Frantisek Foret. 2003. Microdevice for separation and quantitative fraction collection.. https://doi.org/10.1002/elps.200305628

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