Spectral editing and pattern recognition methods applied to high-resolution magic-angle spinning 1H nuclear magnetic resonance spectroscopy of liver tissues.

Yulan Wang; Mary E Bollard; Hector Keun; Henrik Antti; Olaf Beckonert; Timothy M Ebbels; John C Lindon; Elaine Holmes; Huiru Tang; Jeremy K Nicholson

doi:10.1016/j.ab.2003.07.026

PubMed · 14622955

Spectral editing and pattern recognition methods applied to high-resolution magic-angle spinning 1H nuclear magnetic resonance spectroscopy of liver tissues.

Yulan Wang·Mary E Bollard·Hector Keun·Henrik Antti·Olaf Beckonert·Timothy M Ebbels·John C Lindon·Elaine Holmes·Huiru Tang·Jeremy K Nicholson

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Abstract

Principal component analysis (PCA) has been applied to three nuclear magnetic resonance (NMR) spectral editing methods, namely, the Carr-Purcell-Meiboom-Gill spin-echo, diffusion editing, and skyline projection of a two-dimensional J-resolved spectrum, obtained from high-resolution magic-angle spinning NMR spectroscopy of liver tissues, to distinguish between control and hydrazine-treated rats. The effects of the toxin on rat liver biochemistry were directly observed and characterized by depleted levels of liver glycogen, choline, taurine, trimethylamine N-oxide, and glucose and by elevated levels of lipids and alanine. The highly unsaturated omega-3-type fatty acid was observed for the first time in hydrazine-treated rat liver. The contributions of the metabolites to the separation of control from dosed liver tissues varied depending on the type of spectral editing method used. We have shown that subtle changes in the metabolic profiles can be selectively amplified using a metabonomics approach based on the different NMR spectral editing techniques in conjunction with PCA.

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Alanine Animals Chemical and Drug Induced Liver Injury Choline Glucose Glycogen Hydrazines Lipids Liver Liver Diseases Magnetic Resonance Spectroscopy Male Methylamines Rats Rats, Sprague-Dawley Taurine Toxins, Biological Journal Article Research Support, Non-U.S. Gov't

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Yulan Wang, Mary E Bollard, Hector Keun, Henrik Antti, Olaf Beckonert, Timothy M Ebbels, John C Lindon, Elaine Holmes, Huiru Tang, Jeremy K Nicholson. 2003-12-01. Spectral editing and pattern recognition methods applied to high-resolution magic-angle spinning 1H nuclear magnetic resonance spectroscopy of liver tissues.. https://doi.org/10.1016/j.ab.2003.07.026

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