PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 14628739

DNA typing: an important step forward? Collaborative Transplant Study.

G Opelz·J Mytilineos·S Scherer·H Dunckley·J Trejaut·J Chapman·D Middleton·D Savage·G Fischer·J D Bignon·J C Bensa·E Albert·H Noreen
View original source ↗Export metadata ↓

Abstract

In a collaborative project which was supported by 96 transplant centers, DNA typing of HLA-DR antigens was carried out on over 7,000 transplant donors and recipients at 8 participating laboratories. Approximately 25% of the individuals were found to have been typed incorrectly by serological means. An analysis of over 2,500 first cadaver kidney transplants showed a significant correlation of matching for the HLA-DR antigens in transplants where the serological typing was confirmed by DNA typing. In transplants where the serological typing was found to be incorrect, the analysis of serological HLA-DR mismatches resulted in no correlation with graft outcome whereas a significant correlation was found when the corrected DNA typed HLA-DR antigens were analyzed. Transplants which had been reported to the Collaborative Transplant Study based on serological typing as matched for HLA-A, -B, -DR or HLA-B, -DR were found to have a superior graft survival rate only if HLA-DR compatibility was confirmed by DNA typing.

Explore related subjects

DNA FingerprintingGraft SurvivalHLA-DR AntigensHistocompatibility TestingHumansKidney TransplantationTransplantation ImmunologyJournal ArticleMulticenter StudyReview

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

G Opelz, J Mytilineos, S Scherer, H Dunckley, J Trejaut, J Chapman, D Middleton, D Savage, G Fischer, J D Bignon, J C Bensa, E Albert, H Noreen. 1992. DNA typing: an important step forward? Collaborative Transplant Study.. https://doi.org/10.1007/978-3-642-77423-2_169

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2007

Run-specific limits of detection and quantitation for STR-based DNA testing.

STR-based DNA profiling is an exceptionally sensitive analytical technique that is often used to obtain results at the very limits of its sensitivity. The challenge of reliably distinguishing between signal and noise in such situations is one that has been rigorously addressed in numerous other analytical disciplines. However, an inability to determine accurately the height of electropherogram baselines has caused forensic DNA profiling laboratories to utilize alternative approaches. Minimum thresholds established during laboratory validation studies have become the de facto standard for distinguishing between reliable signal and noise/technical artifacts. These minimum peak height thresholds generally fail to consider variability in the sensitivity of instruments, reagents, and the skill of human analysts involved in the DNA profiling process over the course of time. Software (BatchExtract) made publicly available by the National Center for Biotechnology Information now provides an alternative means of establishing limits of detection and quantitation that is more consistent with those used in other analytical disciplines. We have used that software to determine the height of each data collection point for each dye along a control sample's electropherogram trace. These values were then used to determine a limit of detection (the average amount of background noise plus three standard deviations) and a limit of quantitation (the average amount of background noise plus 10 standard deviations) for each control sample. Analyses of the electropherogram data associated with the positive, negative, and reagent blank controls included in 50 different capillary electrophoresis runs validate that this approach could be used to determine run-specific thresholds objectively for use in forensic DNA casework.

DNA Fingerprinting↗