Lactic acid bacteria as prime candidates for codon optimization.

Anders Fuglsang

doi:10.1016/j.bbrc.2003.10.120

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Lactic acid bacteria as prime candidates for codon optimization.

Anders Fuglsang

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Abstract

In species having a strong correlation of expressivity and codon bias it has been shown that heterologous expression can be optimized by changing codons of the introduced gene towards the set of codons that the host organism naturally uses in its highly expressed genes. Even though two lactic acid bacteria are fully sequenced, there are no reports on attempts of codon optimization in the literature. In this report it is demonstrated that codons used in highly expressed genes tend to differ from the codons in lowly expressed genes, and that there is a strong correlation of codon bias and empirical expressivity (codon adaptation index) in Lactococcus lactis and Lactobacillus plantarum. This strongly suggests that codon optimization strategies could be applied to expression systems with lactic acid bacteria as producer strains. A good example of a candidate for codon optimization is the mouse interleukin-2 gene, which in its natural form has an extremely low codon adaptation index for expression in Lc. lactis.

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Algorithms Animals Codon Computer Simulation Gene Expression Profiling Gene Expression Regulation, Bacterial Genetic Variation Interleukin-2 Lactobacillus Lactococcus lactis Mice Models, Genetic Protein Engineering Quality Control Recombinant Proteins Species Specificity Comparative Study Evaluation Study Journal Article

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Anders Fuglsang. 2003-12-12. Lactic acid bacteria as prime candidates for codon optimization.. https://doi.org/10.1016/j.bbrc.2003.10.120

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