Three-dimensional anatomical characterization of the developing mouse brain by diffusion tensor microimaging.

Jiangyang Zhang; Linda J Richards; Paul Yarowsky; Hao Huang; Peter C M van Zijl; Susumu Mori

doi:10.1016/s1053-8119(03)00410-5

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Three-dimensional anatomical characterization of the developing mouse brain by diffusion tensor microimaging.

Jiangyang Zhang·Linda J Richards·Paul Yarowsky·Hao Huang·Peter C M van Zijl·Susumu Mori

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Abstract

Investigation of three-dimensional (3D) morphometry of developing brains has been hindered by a lack of imaging modalities that can monitor the 3D evolution of various anatomical structures without sectioning and staining processes. In this study, we combined magnetic resonance microimaging and diffusion tensor imaging techniques to accomplish such visualization. The application of this approach to developing mouse embryos revealed that it could clearly delineate early critical structures such as neuroepithelium, cortical plate, and various axonal structures, and follow their developmental evolution. The technique was applied to the study of the Netrin-1 mutant, allowing verification of its anatomical phenotype.

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Animals Axons Brain Image Processing, Computer-Assisted Linear Models Magnetic Resonance Imaging Mice Mice, Knockout Models, Neurological Nerve Growth Factors Netrin-1 Neural Pathways Phenotype Tissue Fixation Tumor Suppressor Proteins Journal Article Research Support, U.S. Gov't, P.H.S.

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Jiangyang Zhang, Linda J Richards, Paul Yarowsky, Hao Huang, Peter C M van Zijl, Susumu Mori. 2003. Three-dimensional anatomical characterization of the developing mouse brain by diffusion tensor microimaging.. https://doi.org/10.1016/s1053-8119(03)00410-5

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