Identification of amyloid beta-peptide responsive genes by cDNA microarray technology: involvement of RTP801 in amyloid beta-peptide toxicity.

Jae-Ryong Kim; Seung-Rock Lee; Hyun Jin Chung; Seongyong Kim; Suk-Hwan Baek; Jung Hye Kim; Yong-Sun Kim

doi:10.1038/emm.2003.53

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Identification of amyloid beta-peptide responsive genes by cDNA microarray technology: involvement of RTP801 in amyloid beta-peptide toxicity.

Jae-Ryong Kim·Seung-Rock Lee·Hyun Jin Chung·Seongyong Kim·Suk-Hwan Baek·Jung Hye Kim·Yong-Sun Kim

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Abstract

Amyloid beta-peptide (Abeta), a causative molecule in the pathogenesis of Alzheimer's disease and the main component of senile plaques, is known to be neurotoxic in vitro and in vivo. The mechanisms involved in this Ab-mediated neurotoxicity are not fully understood, although there is evidence to suggest the involvement of oxidative stress, alterations in calcium homeostasis, and/or of CDK activators. Many studies have suggested that Ab may exert its toxic effect via the activation of transcription factors. Therefore, we investigated Ab- responsive genes in human neuroblastoma CHP134 cells using 3.1K human DNA microarrays. Among the several genes overexpressed or repressed by Ab, RTP801, Hi95/sestrin 2, and stanniocalcin 2 were confirmed to be Ab-mediated overexpression in the cells by semiquantitative RT-PCR. Transient expression of the sense RTP801 gene in CHP134 cells increased sensitivity to Abeta cytotoxicity and the expression of the antisense RTP801 gene protected the cells from the Abeta toxicity. These results suggest that RTP801 might play important roles in Abeta toxicity and the pathogenesis of Alzheimer's disease.

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Alzheimer Disease Amyloid beta-Peptides Base Sequence Cell Line, Tumor DNA, Complementary Gene Expression Profiling Gene Expression Regulation Glycoproteins Humans Intercellular Signaling Peptides and Proteins Molecular Sequence Data Nuclear Proteins Oligonucleotide Array Sequence Analysis RNA, Messenger Reverse Transcriptase Polymerase Chain Reaction Transcription Factors Sestrins Journal Article Research Support, Non-U.S. Gov't

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Jae-Ryong Kim, Seung-Rock Lee, Hyun Jin Chung, Seongyong Kim, Suk-Hwan Baek, Jung Hye Kim, Yong-Sun Kim. 2003-10-31. Identification of amyloid beta-peptide responsive genes by cDNA microarray technology: involvement of RTP801 in amyloid beta-peptide toxicity.. https://doi.org/10.1038/emm.2003.53

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