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A cell line that can induce thymocyte positive selection.

P Hugo·J W Kappler·D I Godfrey·P C Marrack
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Abstract

The thymus positively selects thymocytes that bear T-cell receptors which recognize antigen presented by self major histocompatibility complex (MHC) proteins. Positive selection is usually driven by MHC products on radiation-resistant cortical epithelial cells. It is unknown whether positive selection is mediated by all thymic epithelial cells or by some specialized subsets. Here we introduce an H-2b-expressing thymic epithelial cell line into the thymuses of lethally irradiated H-2k animals reconstituted with H-2b/k F1 BM or fetal liver cells. I-Ab-restricted T cells are found in these animals, demonstrating that selection occurs on the introduced epithelial cells.

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AnimalsAntigen-Presenting CellsBone Marrow TransplantationCell LineCells, CulturedClone CellsEnterotoxinsEpitheliumFemaleFetal Tissue TransplantationH-2 AntigensHybridomasInterferon-gammaLiver TransplantationMiceMice, Inbred C57BLMice, Inbred StrainsOvalbuminSpleenStaphylococcus aureusT-LymphocytesThymus GlandJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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P Hugo, J W Kappler, D I Godfrey, P C Marrack. 1992-12-17. A cell line that can induce thymocyte positive selection.. https://doi.org/10.1038/360679a0

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