Amplifiable messenger RNA.

Y Wu; D Y Zhang; F R Kramer

doi:10.1073/pnas.89.24.11769

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Amplifiable messenger RNA.

Y Wu·D Y Zhang·F R Kramer

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Abstract

RNA molecules were prepared that consisted of an mRNA encoding chloramphenicol acetyltransferase embedded within the sequence of midivariant RNA, which is a template for the RNA-directed RNA polymerase Q beta replicase. These recombinant RNAs were shown to be bifunctional: they are amplified exponentially by incubation with Q beta replicase, and the replicated RNA serves as template for the cell-free synthesis of enzymatically active chloramphenicol acetyltransferase. The availability of amplifiable mRNAs will enable relatively large amounts of protein to be synthesized in vitro.

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Base Sequence Chloramphenicol O-Acetyltransferase Genetic Engineering Hydrogen Bonding Molecular Sequence Data Nucleic Acid Conformation Protein Biosynthesis Q beta Replicase RNA, Messenger Transcription, Genetic Journal Article Research Support, U.S. Gov't, P.H.S.

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Y Wu, D Y Zhang, F R Kramer. 1992-12-15. Amplifiable messenger RNA.. https://doi.org/10.1073/pnas.89.24.11769

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