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PubMed · 1466231

Human sperm surface mapping with lectins.

H K Bains·S Sehgal·S R Bawa
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Abstract

Ten fluorescein isothiocyanate (FITC)-linked lectins [Bauhimia purpurea, Concanavalin A, Dolichos biflorus (DBA), Griffonia simplicifolia I, Griffonia simplicifolia II, Maclura pomifera, Arachis hypogea (PNA), Glycine max, Ulex europaeus (UEA) and Triticum vulgaris agglutinin] have been used to study their binding features on the human ejaculate spermatozoa. Qualitative changes in the labeling pattern have been observed in unfixed and acetone-treated spermatozoa. Furthermore, ultrastructural localization of some of the colloidal gold-linked lectins, namely PNA, UEA and DBA, has been attempted to delineate the binding domains of the specific sugars on the sperm surface. It needs to be emphasized that flow-cytometric methods employed in our study, which provide quantitative slant to qualitative data, should be utilized to evaluate the functional status of the spermatozoa.

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Cell MembraneHumansLectinsMaleMicroscopy, ElectronMicroscopy, FluorescenceSpermatozoaJournal Article

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H K Bains, S Sehgal, S R Bawa. 1992. Human sperm surface mapping with lectins.. https://doi.org/10.1159/000147367

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