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PubMed · 14662350

Heterocyst development in Anabaena.

Abstract

Many filamentous nitrogen-fixing cyanobacteria protect nitrogenase from oxygen in differentiated cells called heterocysts. Heterocyst development is controlled by the availability of nitrogen compounds in the environment and by intrinsic factors that regulate the frequency and pattern of heterocysts along vegetative cell filaments. Recent progress in understanding heterocyst development in these simple multicellular organisms includes demonstrating the role of 2-oxoglutarate in regulating the activity of the transcription factor NtcA, the identification of additional genes in the regulatory network, such as hetF, and the further characterization of previously identified genes and proteins, including DevR/HepK, hetR, hetN, patS and patB.

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BibTeXRIS

James W Golden, Ho-Sung Yoon. 2003. Heterocyst development in Anabaena.. https://doi.org/10.1016/j.mib.2003.10.004

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Identification of the glycosyl transferase required for synthesis of the principal glycolipid characteristic of heterocysts of Anabaena sp. strain PCC 7120.

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Target genes for a cAMP receptor protein, AnCrpA, were screened using an Anabaena oligonucleotide microarray and real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. Several gene expressions, including some involved in nitrogen fixation, were downregulated in the ancrpA disruptant when cells were grown with nitrate. Electrophoretic mobility shift assays (EMSAs) revealed that AnCrpA bound to the 5' upstream region of nifB, all1439, hesA, all5347, hglE and coxBII in the presence of cAMP, and all of them are related with nitrogen fixation. A possible AnCrpA-binding site in the 5' upstream region of nifB was predicted using hidden Markov model (HMM) software based on the result of in vitro selection of AnCrpA-binding sequences, and the binding was confirmed by EMSA. Thus, AnCrpA regulates the expressions of gene clusters related to nitrogen fixation in the presence of nitrate.

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