Combining deconvolution and fluctuation analysis to determine quantal parameters and release rates.

Erwin Neher; Takeshi Sakaba

doi:10.1016/j.jneumeth.2003.09.020

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Combining deconvolution and fluctuation analysis to determine quantal parameters and release rates.

Erwin Neher·Takeshi Sakaba

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Abstract

Analysis methods are described which integrate information from fluctuation analysis with that from deconvolution. Together the two approaches allow to derive a consistent quantitative description of quantal release (both evoked, spontaneous and asynchronous) under conditions in which quantal parameters may change during a repetitively applied stimulation protocol. Specifically, our methods take into account the effects of accumulating transmitter in the synaptic cleft and postsynaptic receptor desensitization, which may develop during strong stimulation. Several ways to handle non-stationarities are described. Examples are provided for the Calyx of Held, a glutamatergic synapse, in which both the pre- and the postsynaptic compartments can be voltage-clamped.

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Algorithms Artifacts Auditory Pathways Glutamic Acid Models, Neurological Models, Statistical Neurotransmitter Agents Presynaptic Terminals Receptors, Neurotransmitter Synaptic Membranes Synaptic Transmission Synaptic Vesicles Journal Article Research Support, Non-U.S. Gov't Review

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Erwin Neher, Takeshi Sakaba. 2003-12-15. Combining deconvolution and fluctuation analysis to determine quantal parameters and release rates.. https://doi.org/10.1016/j.jneumeth.2003.09.020

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