Measuring protein self-diffusion in protein-protein mixtures using a pulsed gradient spin-echo technique with WATERGATE and isotope filtering.

Irina V Nesmelova; Djaudat Idiyatullin; Kevin H Mayo

doi:10.1016/j.jmr.2003.09.004

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Measuring protein self-diffusion in protein-protein mixtures using a pulsed gradient spin-echo technique with WATERGATE and isotope filtering.

Irina V Nesmelova·Djaudat Idiyatullin·Kevin H Mayo

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Abstract

Here we report a modified pulsed gradient spin-echo (PGSTE) pulse sequence to measure diffusion coefficients. This approach incorporates WATERGATE combined with isotopic filtering into a standard PGSTE experiment. Doing this eliminates much of the disadvantages from the combination of diffusion encoding and heteronuclear selection intervals and allows for facile modification of the diffusion pulse sequence with flexibility of the time period between RF pulses. The new diffusion pulse sequence is demonstrated using an 15N-labeled peptide and an 15N-labeled protein in a mixture with a protein of similar size.

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Algorithms Complex Mixtures Diffusion Nitrogen Isotopes Nuclear Magnetic Resonance, Biomolecular Peptides Proteins Reproducibility of Results Sensitivity and Specificity Signal Processing, Computer-Assisted Solutions Comparative Study Evaluation Study Journal Article Validation Study

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Irina V Nesmelova, Djaudat Idiyatullin, Kevin H Mayo. 2004. Measuring protein self-diffusion in protein-protein mixtures using a pulsed gradient spin-echo technique with WATERGATE and isotope filtering.. https://doi.org/10.1016/j.jmr.2003.09.004

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