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PubMed · 14680569

[Experimental study on constructing small-caliber artery by tissue engineering approach].

Yang Liu·Yan-zhong Zhang·Jin-jun Chen·De-min Yin·Ying Cao·Zhi-cheng Xu·Wei Liu·Lei Cui·Yi-lin Cao
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Abstract

OBJECTIVE: To investigate the possibility of constructing small-caliber artery by means of tissue engineering. METHODS: Cell-PGA mixtures were made by separately seeding 1 x 10(7) smooth muscle cells and 5 x 10(6) endothelial cells isolated from neonate umbilicus onto PGA scaffold, the cell-PGA constructs were wrapped around a silicone tube before its implantation subcutaneously to nude mice and the mice were sacrificed in 2 and 6 weeks. The tissue engineered artery (TEA) were examined both grossly and immunohistochemically. RESULTS: The gross appearance of TEA was similar to that of the natural counterparts; histologic and immunohistochemical analyses of the neoformed tissues revealed a typical artery structure, including the presence of EC at the luminal surface and the presence of SMC and collagen in the wall. CONCLUSION: TEA with histology similar to natural vessel can be constructed by tissue engineering.

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AnimalsArteriesEndothelial CellsImmunohistochemistryMiceMice, NudeMicroscopy, ElectronMuscle, Smooth, VascularTissue EngineeringEnglish AbstractJournal ArticleResearch Support, Non-U.S. Gov't

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Yang Liu, Yan-zhong Zhang, Jin-jun Chen, De-min Yin, Ying Cao, Zhi-cheng Xu, Wei Liu, Lei Cui, Yi-lin Cao. 2003. [Experimental study on constructing small-caliber artery by tissue engineering approach].. https://pubmed.ncbi.nlm.nih.gov/14680569/

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