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Cellular requirements for CRM1 import and export.

Xianfeng Zhang·Masami Yamada·Naoto Mabuchi·Hisatoshi Shida
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Abstract

We studied the cellular requirements for the translocation of CRM1 (exportin 1) between the nucleus and the cytoplasm. CRM1 import requires neither ATP, Ran, Ran-dependent GTP hydrolysis, nor a particular temperature. CRM1 and importin beta compete with each other during their import. Thus, CRM1 is able to enter the nucleus in a manner similar to importinbeta. In contrast, the in vivo export of CRM1 involves ATP-consuming step(s).

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Active Transport, Cell NucleusAdenosine TriphosphateBinding, CompetitiveCell LineCell NucleusCytoplasmHumansHydrolysisKaryopherinsReceptors, Cytoplasmic and NuclearTemperaturebeta Karyopherinsran GTP-Binding ProteinExportin 1 ProteinJournal Article

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Xianfeng Zhang, Masami Yamada, Naoto Mabuchi, Hisatoshi Shida. 2003. Cellular requirements for CRM1 import and export.. https://doi.org/10.1093/jb%2Fmvg197

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