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PubMed · 14700559

A second-generation copper(II)-mediated metallo-DNA-base pair.

Nicole Zimmermann·Eric Meggers·Peter G Schultz
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Abstract

Metal-dependent pairing of nucleobases represents an alternative DNA base pairing scheme. Our first-generation copper(II)-mediated pyridine-2,6-dicarboxylate (Dipic) and pyridine (Py) metallo-base pair has a stability comparable to the natural base pairs dA:dT and dC:dG but does not have the selectivity of the Watson Crick base pairs. In order to increase the selectivity of base pair formation, a second-generation metallo-base pair was generated consisting of a pyridine-2,6-dicarboxamide (Dipam) and a pyridine (Py) nucleobase. This new metallo-base pair is more stable than the natural base pairs dA:dT and dC:dG and highly selective against mispairing. In addition, incorporation of multiple metallo-base pairs into DNA results in the formation of stable duplexes demonstrating that hydrogen bonding base pairs can efficiently be replaced by metal-dependent base pairs at multiple sites in DNA.

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Base PairingCopperDNALigandsNucleic Acid ConformationNucleosidesOrganometallic CompoundsOrganophosphorus CompoundsPicolinic AcidsPyridinesTemperatureJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Nicole Zimmermann, Eric Meggers, Peter G Schultz. 2004. A second-generation copper(II)-mediated metallo-DNA-base pair.. https://doi.org/10.1016/j.bioorg.2003.09.001

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