Vanadate and insulin stimulate gene 33 expression.

R S Weinstock; J L Messina

doi:10.1016/0006-291x(92)92293-7

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Vanadate and insulin stimulate gene 33 expression.

R S Weinstock·J L Messina

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Abstract

Vanadate and insulin stimulated gene 33 expression in rat H4 hepatoma cells. Vanadate (10(-3) M) maximally increased gene 33 transcription 4-fold at 15 min. The maximal insulin induction (3-4-fold) was observed using 5 x 10(-9) M insulin for 30 min. Both vanadate and insulin increased cytoplasmic mRNA levels in a dose-dependent manner, with maximal effects observed by 60 min. These effects on gene 33 mRNA accumulation were greater than those on transcription. Vanadate (10(-3) M) maximally stimulated gene 33 mRNA levels 10-12-fold. The vanadate and insulin effects were not synergistic. Thus, high concentrations of vanadate regulate gene 33 expression in an insulin-like manner and, like insulin, vanadate probably controls transcriptional as well as post-transcriptional events.

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Animals Cytoplasm Dose-Response Relationship, Drug Gene Expression Insulin Kinetics Liver Neoplasms, Experimental RNA, Messenger Rats Transcription, Genetic Tumor Cells, Cultured Vanadates Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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R S Weinstock, J L Messina. 1992-12-15. Vanadate and insulin stimulate gene 33 expression.. https://doi.org/10.1016/0006-291x(92)92293-7

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