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PubMed · 14724094

[Preparation of the microarray for detecting hepatitis D virus].

Zhao-hui Sun·Wen-ling Zheng·Bao Zhang·Rong Shi·Wen-li Ma
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Abstract

OBJECTIVE: To prepare the microarray for detection of hepatitis D virus (HDV). METHOD: Several pairs of specific PCR primers were designed according to the conserved region of HDV genome. The DNA microarray were prepared by blotting the PCR products onto the surface of glass slides with the use of robotics, and restriction display PCR (RD-PCR) was employed to label the samples. RESULT: Sequences analysis showed that the products of PCR amplification were the specific gene fragments of HDV. Hybridization signals on the gene chip demonstrated good specificity and sensitivity of the microarray for HDV detection. CONCLUSION: Microarray-based clinical HDV detection can be sensitive and effective.

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Base SequenceHepatitis Delta VirusMolecular Sequence DataOligonucleotide Array Sequence AnalysisPolymerase Chain ReactionRNA, ViralEnglish AbstractJournal ArticleResearch Support, Non-U.S. Gov't

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Zhao-hui Sun, Wen-ling Zheng, Bao Zhang, Rong Shi, Wen-li Ma. 2004. [Preparation of the microarray for detecting hepatitis D virus].. https://pubmed.ncbi.nlm.nih.gov/14724094/

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