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Sponge-cell culture? A molecular identification method for sponge cells.

Detmer Sipkema·Hans G H J Heilig·Antoon D L Akkermans·Ronald Osinga·Johannes Tramper·René H Wijffels
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Abstract

Dissociated sponge cells are easily confused with unicellular organisms. This has been an obstacle in the development of sponge-cell lines. We developed a molecular detection method to identify cells of the sponge Dysidea avara in dissociated cell cultures. The 18S ribosomal RNA gene from a Dysidea avara specimen was sequenced and compared to eukaryotic 18S rDNA sequences picked up from a proliferating cell culture that originated from a dissociated Dysidea avara specimen. Our method proved unambiguously that this was not a sponge-cell culture. Therefore, it provides a valuable tool for further research on sponge-cell cultures.

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AnimalsBase SequenceCell Culture TechniquesDNA PrimersGenetic VariationMolecular Sequence DataPolymorphism, Restriction Fragment LengthPoriferaRNA, RibosomalSequence Analysis, DNAComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Detmer Sipkema, Hans G H J Heilig, Antoon D L Akkermans, Ronald Osinga, Johannes Tramper, René H Wijffels. Sponge-cell culture? A molecular identification method for sponge cells.. https://doi.org/10.1007/s10126-002-0090-1

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