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Concatenation cDNA sequencing for transcriptome analysis.

Preethi H Gunaratne·Jia Qian Wu·Angela M Garcia·Steven Hulyk·Kim C Worley·Judith F Margolin·Richard A Gibbs
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Abstract

We describe a high-throughput cDNA sequencing pipeline (http://www.hgsc.bcm.tmc.edu/projects/cdna) built in response to the emerging need for rapid sequencing of large cDNA collections. Using this strategy cDNA inserts are purified and joined through concatenation into large molecules. These 'pseudo-BACs' are subjected to random shotgun sequencing whereby the majority of cDNA inserts in the pool are sequenced. Using this concatenation cDNA sequencing platform, we have contributed more than 13000 full-length cDNA sequences from human and mouse to the Mammalian Gene Collection (MGC).

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Cloning, MolecularDNA, ComplementaryDNA, ConcatenatedGenetic TechniquesHumansSequence Analysis, DNATranscription, GeneticJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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Preethi H Gunaratne, Jia Qian Wu, Angela M Garcia, Steven Hulyk, Kim C Worley, Judith F Margolin, Richard A Gibbs. Concatenation cDNA sequencing for transcriptome analysis.. https://doi.org/10.1016/j.crvi.2003.09.032

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