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PubMed · 14745965

Early requirement for fgf8 function during hindbrain pattern formation in zebrafish.

Elizabeth L Wiellette·Hazel Sive
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Abstract

Fibroblast growth factor (FGF) signaling is required for normal development of the vertebrate brain, including the isthmus and caudal regions of the hindbrain. Recent work in zebrafish has identified a requirement for the combination of fgf3 and fgf8 functions in specification of rhombomeres 5 and 6 (r5, r6), when evaluated at mid- and late somitogenesis stages. However, when examined earlier in development, during early somitogenesis stages, FGF8 alone is required to initiate r5 and r6 development. Both a mutation in fgf8 and injection of fgf8-targeted antisense morpholino-modified oligonucleotides result in suppression of genes normally expressed in r5 and r6 by the one- to two-somite stage. This expression recovers by the six-somite stage, and we propose that this recovery is a response to activation of fgf3 and to delayed accumulation of fgf8. These data demonstrate an early, nonredundant requirement for fgf8 function in hindbrain patterning.

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AnimalsBody PatterningEmbryo, NonmammalianFibroblast Growth Factor 3Fibroblast Growth Factor 8Fibroblast Growth FactorsGene Expression Regulation, DevelopmentalIn Situ HybridizationMorphogenesisOligonucleotides, AntisenseRhombencephalonSignal TransductionSomitesZebrafishZebrafish ProteinsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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Elizabeth L Wiellette, Hazel Sive. 2004. Early requirement for fgf8 function during hindbrain pattern formation in zebrafish.. https://doi.org/10.1002/dvdy.10464

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