[Basic principles of the antisense strategy].

Roxana Georgiana Tauşer; Ortansa Stoica

PubMed · 14756049

[Basic principles of the antisense strategy].

Abstract

The antisens strategy is based on specific inhibition of the mutant gene expression by oligodeoxynucleotides (OGN), capable of selectively hybridizing with target DNA or RNA. Molecular mechanisms of the antisense oligonucleotide comprise: inhibition of splicing, inhibition of 5'-capping and 3'-polyadenylation, activation of RN-ase H, small interfering RNA, ribozymes. The antisense strategy is applied to: rational design of potent, selective therapeutic agents, target validation and detection of pathologic gene expression in vivo. The main aim of the antisense oligonucleotide design is to improve the affinity for target RNA and the resistance to nucleolytic degradation.

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Enzyme Activation Gene Expression Regulation Humans Mutation Oligodeoxyribonucleotides, Antisense Polyadenylation RNA Splicing RNA, Catalytic RNA, Ribosomal RNA, Small Interfering Ribonuclease H English Abstract Journal Article Review

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Roxana Georgiana Tauşer, Ortansa Stoica. [Basic principles of the antisense strategy].. https://pubmed.ncbi.nlm.nih.gov/14756049/

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